ScisGo-HLA-v6 is a next generation sequencing (NGS) technology that yields high resolution genotypes for all eleven HLA-class I (CI) and class II (CII) loci. The NGS data cover all exon and sufficient intron sequences to yield 3-field typing with detection of all known null alleles. Ambiguities are minimized with zero allele ambiguities and rare diploid ambiguities encountered at the 3-field HLA allele level. We offer range of standard and customized data reporting options.
ScisGo-KIR-v3 is a system that allows for the determination of gene copy number and haplotype as well as targeted KIR gene allele determination. Gene copy number is determined by comparisons of amplicon-derived read numbers between distinct KIR exon/loci. Haplotypes are deduced from gene copy number according to established reference haplotypes.
ScisGo-MICAB-v3 is a next generation sequencing (NGS) technology that yields high resolution genotypes for MICA and MICB. The NGS data cover all exon and sufficient intron sequences to yield 2-field typing for all loci including the detection of all known null alleles.
ScisGo-FcR-IGHG technology utilizes existing data sets at the IMGT and the NCBI genbank repository supplemented with long range sequencing of 1000 genome samples from 25 populations providing a comprehensive database of FcR and IGHG polymorphism. This enhanced database allows the majority of variants detected by the short-read NGS assay to be phased accordingly through mapping back to the database. Our current targeting probes for the FcR genes include a panel of variants that have been previously characterized functionally. The four IgG encoding genes are targeted at the CH1, CH2 and CH3 regions